Together such changes lead to modulation of host proteome and transcriptome thereby leading to major alterations in signal transduction that favor invasion and bacterial multiplication. Quantification of punctate PERP staining in response to Salmonella infection. Science. However, recombinant GroELs S4. Our lab utilises murine models, organotypic models and human patient samples to carry out these investigations. Published by The Company of Biologists Ltd.Competing interestsThe authors declare no competing or financial interests.This site needs JavaScript to work properly. Still others such as Ehrlichia chaffeensis and Anaplasma phagocytophilum utilize host SUMO-machinery for sumoylating their own effectors. In future we aim to dissect the precise role of PTM pathways in governing epithelial-immune crosstalk particularly in the context of intestinal inflammation. 1999 Aug 16;18(16):4394-403. doi: 10.1093/emboj/18.16.4394.Verma S, Mohapatra G, Ahmad SM, Rana S, Jain S, Khalsa JK, Srikanth CV.Mol Cell Biol. Uncontrolled inflammation contributes to the pathogenesis of inflammatory diseases as well as the development of cancers. Five micron cryosections of mouse proximal colon were stained for PERP (green) or DAPI (blue). Se você é um Usuário Master e este é seu primeiro acesso, clique aqui. Using a mouse model of this condition, the authors demonstrated previously that the cytokine gamma interferon (IFN-gamma) is required for a normal intestinal inflammatory response to the pathogen. All rights reserved.Correction for Kumar et al., "Facilitated Oligomerization of Mycobacterial GroEL: Evidence for Phosphorylation-Mediated Oligomerization",A SUMOylation-dependent switch of RAB7 governs intracellular life and pathogenesis of Salmonella Typhimurium,SUMOylation pathway alteration coupled with downregulation of SUMO E2 enzyme at mucosal epithelium modulates inflammation in inflammatory bowel disease,Sumoylation as an Integral Mechanism in Bacterial Infection and Disease Progression,Understanding the Complexities of Salmonella-Host Crosstalk as Revealed by In Vivo Model Organisms,Salmonella Engages Host MicroRNAs To Modulate SUMOylation: a New Arsenal for Intracellular Survival.PERP, a Host Tetraspanning Membrane Protein, is Required for Salmonella-Induced Inflammation.Salmonella effector proteins and host-cell responses,Targeting Tumors with Salmonella Typhimurium - Potential for Therapy.Srikanth CV, Wall DM, Maldonado-Contreras A, Shi HN, Zhou D, Demma Z, Mumy KL, McCormick BA. It is classified as a member of the heat shock protein family Hsp60 that essentially form isologous tetradecameric rings with two central cavities for ATP dependent binding and release of unfolded, misfolded or kinet...I am interested in understanding the structure - function attributes of chaperonins.Join ResearchGate to find the people and research you need to help your work.Only verified researchers can join ResearchGate and send messages to other members.University students and faculty, institute members, and independent researchers,Technology or product developers, R&D specialists, and government or NGO employees in scientific roles,Health care professionals, including clinical researchers,Journalists, citizen scientists, or anyone interested in reading and discovering research.Sorry, you need to be a researcher to join ResearchGate.Due to our privacy policy, only current members can send messages to people on ResearchGate.© 2008-2020 ResearchGate GmbH. REGIONAL CENTRE FOR BIOTECHNOLOGY Journal Club CV Srikanth, PhD Wednesday, 30 th April, 2014 ,4.00 PM Seminar room “FXR is a molecular target for the effects of … Mohapatra G(1)(2), Gaur P(1), Mujagond P(1), Singh M(3), Rana S(1)(2), Pratap S(1), Kaur N(4), Verma S(5), Krishnan V(1), Singh N(1), Srikanth CV(6). The level of PERP punctate staining was quantified with FIJI using the same size filter for the punctae across all samples. Expression of PERP in mouse colon. Srikanth CV, Verma S (2017) Sumoylation as an Integral Mechanism in Bacterial Infection and Disease Progression. Consistent with this idea, we found that IDO-deficient mic...The distinctive feature of the GroES-GroEL chaperonin system in mediating protein folding lies in its ability to exist in a tetradecameric state, form a central cavity and encapsulate the substrate via GroES lid. 2015 Sep 1;35(17):2932-46. doi: 10.1128/MCB.00397-15. Understandably, bacterial pathogens intervene with the SUMO pathway of the host for ensuring successful infection. S1. COVID-19 is an emerging, rapidly evolving situation.Post translational modification pathways regulate fundamental processes of cells and thus govern vital functions. Initially in a murine model of...Post translational modification pathways regulate fundamental processes of cells and thus govern vital functions. Among the bacterial pathogens known to target host sumoylation varied points of intervention are utilized. CD and UC are GI autoimmine disorders with very high incidence in developing and developed nations.Using state of the art tools of microbiology, molecular biology and fluorescent-imaging, proteomics we intend to investigate complex mechanisms operational in the gut. 2010 Nov-Dec;1(6):532-4. doi: 10.4161/viru.1.6.13449. S5. 2006 Jul-Sep;2(3):156-8. doi: 10.4161/auto.2825. Centre for DNA Fingerprinting and Diagnostics (CDFD), Hyderabad; Center of Innovative and Applied Bioprocessing (CIAB), Mohali; National Institute of Animal Biotechnology (NIAB), Hyderabad