Both use carbolfuchsin as the primary stain. The use of acid-alcohol in the technique earned it the name Acid-Fast Stain and the application of heat in the technique gives it the name the hot method of Acid-Fast staining which is a synonymous name for the Ziehl-Neelsen Staining technique. Yield of microscopy is dependent on stain selection because the auramine-rhodamine fluorescent stain is more sensitive than the traditional Kinyoun or Ziehl–Neelsen stains. © 2003-2020 Chegg Inc. All rights reserved. Components of Acid-Fast Stain. Most common of the acid fast staining techniques is the Ziehl-Neelsen technique. | What is the usual decolorizer in the Ziehl-Neelsen acid-fast stain procedure? These Ziehl-Neelsen stained cells are ..Acid-fast positive b. Acid-fast negative 28. The Ziehl-Neelsen stain (ZN stain), also called the hot method of AFB staining, is a type of differential bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria.. These Ziehl-Neelsen stained cells are ..Acid-fast positive b. Acid-fast negative 28. Acid-fastness is a physical property of certain bacterial and eukaryotic cells, as well as some sub-cellular structures, specifically their resistance to decolorization by acids during laboratory staining procedures. & What is your next step? What is the primary stain in the Shaeffer-Fulton endospore stain procedure? View desktop site, Ziehl-Neelsen stain The Ziehl-Neelsen stain (ZN stain), also called the hot method of Acid fast Bacilli staining. Water clodine . This technique is useful for the identification of oocysts of the coccidian species (Cryptosporidium, Cystoisospora, and Cyclospora), which may be difficult to detect with routine stains such as trichrome.Unlike the Ziehl-Neelsen Modified Acid-Fast Stain, this stain does not require the heating of reagents for staining. Add acid-alcohol. These acids resist staining by ordinary methods such as a Gram stain. Staining procedure: Acid-fast organisms like Mycobacterium contain large amounts of lipid substances within their cell walls called mycolic acids. Principle of acid fast staining (in mycobacteria): The waxy mycolic acid containing cell wall of mycobacteia are relatively impermeable to ordinary staining techniques. It can also be used to stain a few other bacteria, such as Nocardia. Once stained as part of a sample, these organisms can resist the acid and/or ethanol-based decolorization procedures common in many staining protocols, hence the name acid-fast. Acid fast organisms are those which are capable of retaining the primary stain when treated with an acid (fast=holding capacity).Members of the Actinomycetes, genus Nocardia (N. brasiliensis and N. … Malachite green Safranin 2. a Carbolfuscin Crystal violet c. Methylene blue . Detection of acid-fast bacilli (AFB) in stained and acid-washed smears examined microscopically may provide the initial bacteriologic evidence of the presence of mycobacteria in a clinical specimen. Acid-fast staining is a differential staining procedure, which uses the … Acid-fast staining and microscopic examination are the easiest, quickest, and least expensive diagnostic procedures. While performing the acid-fast stain procedure, you heat your fixed bacteria with carbolfuchsin for 5 minutes, let the slide cool, and then rinse with water. Examples of acid-fast bacteria: Mycobacterium tuberculosis, M. leprae, M. smegmatis, M. phlei etc. A smear from a mixed culture of bacilli and cocci is stained with the acid-fast procedure. Malachite green Safranin 2. What is the usual decolorizer in the Ziehl-Neelsen acid-fast stain procedure? Acid-alcohol Ethyl alcohol 2s. Tuberculosis, M. smegmatis, M. phlei etc more sensitive than the Kinyoun... 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